Deep sequencing of small RNA facilitates tissue and sex associated microRNA discovery in zebrafish.

ABSTRACT:

Background

The role of microRNAs in gene regulation has been well established. The extent of miRNA regulation also increases with increasing genome complexity. Though the number of genes appear to be equal between human and zebrafish, substantially less microRNAs have been discovered in zebrafish compared to human (miRBase Release 19). It appears that most of the miRNAs in zebrafish are yet to be discovered.

Results

We sequenced small RNAs from brain, gut, liver, ovary, testis, eye, heart and embryo of zebrafish. In brain, gut and liver sequencing was done sex specifically. Majority of the sequenced reads (16-62 %) mapped to known miRNAs, with the exception of ovary (5.7 %) and testis (7.8 %). Using the miRNA discovery tool (miRDeep2), we discovered novel miRNAs from the una

SUBMITTER: Vaz C 

PROVIDER: S-EPMC4647824 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature