OSU-CG5, a novel energy restriction mimetic agent, targets human colorectal cancer cells in vitro.

ABSTRACT:

Aim

Energy-restriction mimetic agents (ERMAs) are small-molecule agents that target various aspects of energy metabolism, which has emerged as a promising approach in cancer therapy. In the current study, we tested the ability of OSU-CG5, a novel ERMA, to target human colorectal cancer (CRC) in vitro.

Methods

Two human CRC cell lines (HCT-116 and Caco-2) were tested. Cell viability was assessed using MTT assay. Caspase-3/7 activities were measured using Caspase-Glo 3/7 assay kit. Western blot analysis was used to measure the expression of relevant proteins in the cells. Glucose consumption of the cells was detected using glucose uptake cell-based assay kit.

Results

OSU-CG5 dose-dependently inhibited HCT-116 and Caco-2 cell proliferation with the IC₅₀ values of 3.9 and

SUBMITTER: Arafa el-SA 

PROVIDER: S-EPMC4647897 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature