Superresolution imaging with optical fluctuation using speckle patterns illumination.

ABSTRACT: Superresolution fluorescence microscopy possesses an important role for the study of processes in biological cells with subdiffraction resolution. Recently, superresolution methods employing the emission properties of fluorophores have rapidly evolved due to their technical simplicity and direct applicability to existing microscopes. However, the application of these methods has been limited to samples labeled with fluorophores that can exhibit intrinsic emission properties at a restricted timescale, especially stochastic blinking. Here, we present a superresolution method that can be performed using general fluorophores, regardless of this intrinsic property. Utilizing speckle patterns illumination, temporal emission fluctuation of fluorophores is induced and controlled, from which a supe

SUBMITTER: Kim M 

PROVIDER: S-EPMC4648106 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature