Intestinal absorption characteristics of imperialine: in vitro and in situ assessments.

ABSTRACT:

Aim

Imperialine is an effective compound in the traditional Chinese medicine chuanbeimu (Bulbus Fritillariae Cirrhosae) that has been used as antitussive/expectorant in a clinical setting. In this study we investigated the absorption characteristics of imperialine in intestinal segments based on an evaluation of its physicochemical properties.

Methods

Caco-2 cells were used to examine uptake and transport of imperialine in vitro, and a rat in situ intestinal perfusion model was used to characterize the absorption of imperialine. The amount of imperialine in the samples was quantified using LC-MS/MS.

Results

The aqueous solubility and oil/water partition coefficient of imperialine were determined. This compound demonstrated a relatively weak alkalinity with a pKa of 8.

SUBMITTER: Lin Q 

PROVIDER: S-EPMC4648119 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature