SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy.

ABSTRACT: Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.

SUBMITTER: Ball G 

PROVIDER: S-EPMC4648340 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature