Analysing calcium signalling of cells under high shear flows using discontinuous dielectrophoresis.

ABSTRACT: Immobilisation of cells is an important feature of many cellular assays, as it enables the physical/chemical stimulation of cells; whilst, monitoring cellular processes using microscopic techniques. Current approaches for immobilising cells, however, are hampered by time-consuming processes, the need for specific antibodies or coatings, and adverse effects on cell integrity. Here, we present a dielectrophoresis-based approach for the robust immobilisation of cells, and analysis of their responses under high shear flows. This approach is quick and label-free, and more importantly, minimises the adverse effects of electric field on the cell integrity, by activating the field for a short duration of 120 s, just long enough to immobilise the cells, after which cell culture media (such as HEPES

SUBMITTER: Soffe R 

PROVIDER: S-EPMC4648442 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature