High-Throughput Intracellular Antimicrobial Susceptibility Testing of Legionella pneumophila.

ABSTRACT: Legionella pneumophila is a Gram-negative opportunistic human pathogen that causes a severe pneumonia known as Legionnaires' disease. Notably, in the human host, the organism is believed to replicate solely within an intracellular compartment, predominantly within pulmonary macrophages. Consequently, successful therapy is predicated on antimicrobials penetrating into this intracellular growth niche. However, standard antimicrobial susceptibility testing methods test solely for extracellular growth inhibition. Here, we make use of a high-throughput assay to characterize intracellular growth inhibition activity of known antimicrobials. For select antimicrobials, high-resolution dose-response analysis was then performed to characterize and compare activity levels in both macrophage infection

SUBMITTER: Chiaraviglio L 

PROVIDER: S-EPMC4649152 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature