Elucidating the Role of Residue 67 in IMP-Type Metallo-β-Lactamase Evolution.

ABSTRACT: Antibiotic resistance in bacteria is ever changing and adapting, as once-novel β-lactam antibiotics are losing their efficacy, primarily due to the production of β-lactamases. Metallo-β-lactamases (MBLs) efficiently inactivate a broad range of β-lactam antibiotics, including carbapenems, and are often coexpressed with other antibacterial resistance factors. The rapid dissemination of MBLs and lack of novel antibacterials pose an imminent threat to global health. In an effort to better counter these resistance-conferring β-lactamases, an investigation of their natural evolution and resulting substrate specificity was employed. In this study, we elucidated the effects of different amino acid substitutions at position 67 in IMP-type MBLs on the ability to hydrolyze and confer resistance to a

SUBMITTER: LaCuran AE 

PROVIDER: S-EPMC4649200 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature