High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM.

ABSTRACT: Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause major changes to the light path resulting in loss of signal and contrast in the deepest regions, thus impairing in-depth imaging capability. These effects are particularly marked when inhomogeneous, complex biological samples are under study. Recently, chemical treatments have been developed to render a sample transparent by homogenizing its refractive index (RI), consequently enabling a reduction of scattering phenomena and a simplification of optical aberration patterns. One drawback of these methods

SUBMITTER: Masson A 

PROVIDER: S-EPMC4649629 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature