Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection.

ABSTRACT:

Background

Leishmaniasis is a neglected tropical disease that is caused by an obligate intracellular protozoan of the genus Leishmania. Recently, an increasing number of autochthonous leishmaniasis cases caused by L. martiniquensis and the novel species L. siamensis have been described in Thailand, rendering an accurate diagnosis of this disease critical. However, only a few laboratories are capable of diagnosing leishmaniasis in Thailand. To expand leishmaniasis diagnostic capabilities, we developed a simple colorimetric loop-mediated isothermal amplification (LAMP) technique for the direct detection of Leishmania DNA.

Methods

LAMP was performed for 75 min using four primers targeting the conserved region of the18S ribosomal RNA gene, and the DNA indicator used was malachit

SUBMITTER: Sriworarat C 

PROVIDER: S-EPMC4650110 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature