MiR-449a targets Flot2 and inhibits gastric cancer invasion by inhibiting TGF-β-mediated EMT.

ABSTRACT:

Background

Flot2, a highly conserved protein of the SPFH domain containing proteins family, has recently been identified as oncogene to be involved in the tumorigenesis and metastasis of several cancers including gastric cancer. However, the underlying molecular mechanism of Flot2 in gastric cancer (GC) is largely unknown.

Methods

qRT-PCR and western blot was performed to detect miR-449a and Flot2 expression in GC cell lines and Normal human gastric epithelial cells. Then, luciferase reporter assay was used to elucidate whether Flot2 is a target gene of miR-449a. Finally, the roles and mechanism of miR-449a in regulation of tumor invasion were further investigated.

Results

In this study, miR-449a expression was downregulated and Flot2 was upregulated in all GC cell li

SUBMITTER: Li Q 

PROVIDER: S-EPMC4650491 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature