Identification of Enterococcus faecalis antigens specifically expressed in vivo.

ABSTRACT:

Objectives

Molecular mechanism of the pathogenicity of Enterococcus faecalis (E. faecalis), a suspected endodontic pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. Here we report the identification of in vivo expressed antigens of E. faecalis by using a novel immunoscreening technique called change-mediated antigen technology (CMAT) and an experimental animal model of endodontic infection.

Materials and methods

Among 4,500 E. coli recombinant clones screened, 19 positive clones reacted reproducibly with hyperimmune sera obtained from rabbits immunized with E. faecalis cells isolated from an experimental endodontic infection. DNA sequences from 16 of these in vivo-induced (IVI) genes were determined.

Results

Identifi

SUBMITTER: Lee SW 

PROVIDER: S-EPMC4650527 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature