Alzheimer's disease-related amyloid-β induces synaptotoxicity in human iPS cell-derived neurons.

ABSTRACT: Human induced pluripotent stem cell (iPSC)-derived neurons have been proposed to be a highly valuable cellular model for studying the pathomechanisms of Alzheimer's disease (AD). Studies employing patient-specific human iPSCs as models of familial and sporadic forms of AD described elevated levels of AD-related amyloid-β (Aβ). However, none of the present AD iPSC studies could recapitulate the synaptotoxic actions of Aβ, which are crucial early events in a cascade that eventually leads to vast brain degeneration. Here we established highly reproducible, human iPSC-derived cortical cultures as a cellular model to study the synaptotoxic effects of Aβ. We developed a highly efficient immunopurification procedure yielding immature neurons that express markers of deep layer cortical pyramidal n

SUBMITTER: Nieweg K 

PROVIDER: S-EPMC4650541 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature