Quantitative assessment of single-cell whole genome amplification methods for detecting copy number variation using hippocampal neurons.

ABSTRACT: Single-cell genomic analysis has grown rapidly in recent years and finds widespread applications in various fields of biology, including cancer biology, development, immunology, pre-implantation genetic diagnosis, and neurobiology. To date, the amplification bias, amplification uniformity and reproducibility of the three major single cell whole genome amplification methods (GenomePlex WGA4, MDA and MALBAC) have not been systematically investigated using mammalian cells. In this study, we amplified genomic DNA from individual hippocampal neurons using three single-cell DNA amplification methods, and sequenced them at shallow depth. We then systematically evaluated the GC-bias, reproducibility, and copy number variations among individual neurons. Our results showed that single-cell genome se

SUBMITTER: Ning L 

PROVIDER: S-EPMC4650676 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature