Sequencing-based high throughput mutation detection in bread wheat.

ABSTRACT:

Background

Forward genetic approaches have limited use for agronomic traits that can't be reliably scored on a single plant basis. Thus, mutants in wheat and other crops are more useful for gene function studies by reverse genetic approach. With a long-term goal to develop a sequence-based mutation detection resource in hexaploid wheat, we conducted a feasibility study to accurately differentiate induced mutations from the homoeologs' sequence variations present among the three wheat genomes.

Results

A reduced representation ApeKI library consisting of 21 Ethylmethane Sulfonate (EMS) induced mutants and two wild type cv. Indian plants was developed using individual barcode adapters and sequenced. A novel bioinformatics pipeline was developed to identify sequence variants usi

SUBMITTER: Sidhu G 

PROVIDER: S-EPMC4650848 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature