Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer.

ABSTRACT:

Background

Tumour cells and stromal cells interact in the tumour microenvironment; moreover, stromal cells can acquire abnormalities that contribute to tumour progression. However, interactions between lymphatic endothelial cells (LECs) and tumour cells are largely unexamined. In this study, we aimed to determine whether tumour-specific LECs inhabit the tumour microenvironment and examine their influence on this microenvironment.

Methods

We isolated normal LECs (NLECs) from a non-metastatic lymph node and tumour-associated LECs (TLECs) from cancerous lymph nodes. We examined proliferative and migratory potency, growth factor production, and gene expression of each type of LEC. Moreover, we developed a co-culture system to investigate the interactions between gastric cancer c

SUBMITTER: Tokumoto MW 

PROVIDER: S-EPMC4651131 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature