Potential of DNA methylation in rectal cancer as diagnostic and prognostic biomarkers.

ABSTRACT:

Background

Aberrant DNA methylation is more prominent in proximal compared with distal colorectal cancers. Although a number of methylation markers were identified for colon cancer, yet few are available for rectal cancer.

Methods

DNA methylation differences were assessed by a targeted DNA microarray for 360 marker candidates between 22 fresh frozen rectal tumour samples and 8 controls and validated by microfluidic high-throughput and methylation-sensitive qPCR in fresh frozen and formalin-fixed paraffin-embedded (FFPE) samples, respectively. The CpG island methylator phenotype (CIMP) was assessed by MethyLight in FFPE material from 78 patients with pT2 and pT3 rectal adenocarcinoma.

Results

We identified and confirmed two novel three-gene signatures in fresh frozen s

SUBMITTER: Exner R 

PROVIDER: S-EPMC4651135 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature