A Microfluidic Device for Multiplex Single-Nucleotide Polymorphism Genotyping.

ABSTRACT: Single-nucleotide polymorphisms (SNPs) are the most abundant type of genetic variations; they provide the genetic fingerprint of individuals and are essential for genetic biomarker discoveries. Accurate detection of SNPs is of great significance for disease prevention, diagnosis and prognosis, and for prediction of drug response and clinical outcomes in patients. Nevertheless, conventional SNP genotyping methods are still limited by insufficient accuracy or labor-, time-, and resource-intensive procedures. Microfluidics has been increasingly utilized to improve efficiency; however, the currently available microfluidic genotyping systems still have shortcomings in accuracy, sensitivity, throughput and multiplexing capability. To address these challenges, we developed a multi-step SNP genoty

SUBMITTER: Zhu J 

PROVIDER: S-EPMC4651459 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature