HDACs Regulate miR-133a Expression in Pressure Overload-Induced Cardiac Fibrosis.

ABSTRACT:

Background

MicroRNAs (miRNAs) and histone deacetylases (HDACs) serve a significant role in the pathogenesis of a variety of cardiovascular diseases. The transcriptional regulation of miRNAs is poorly understood in cardiac hypertrophy. We investigated whether the expression of miR-133a is epigenetically regulated by class I and IIb HDACs during hypertrophic remodeling.

Methods and results

Transverse aortic constriction (TAC) was performed in CD1 mice to induce pressure overload hypertrophy. Mice were treated with class I and IIb HDAC inhibitor (HDACi) via drinking water for 2 and 4 weeks post TAC. miRNA expression was determined by real-time polymerase chain reaction. Echocardiography was performed at baseline and post TAC end points for structural and functional assessment.

SUBMITTER: Renaud L 

PROVIDER: S-EPMC4651803 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature