The original Beckman Coulter Generation II assay significantly underestimates AMH levels compared with the revised protocol.

ABSTRACT:

Purpose

Anti-Müllerian hormone (AMH) is used as a marker for ovarian reserve. Since 2011, the standard test for AMH has been the Beckman Coulter Generation (Gen) II assay. However, in July 2013, the protocol was revised due to falsely low readings. The aim of this study was to compare AMH levels measured with the original and revised Gen II assay and to establish a fertile female reference range for the revised protocol.

Methods

Serum AMH levels were measured for 492 natural conception first trimester pregnant women using the original and revised Gen II assay.

Results

The original protocol significantly underestimated AMH levels compared with the revised protocol (p < 0.001), the median being 8.4 and 14.2 pmol/L, respectively. In all samples with detectable AMH levels

SUBMITTER: Bonifacio M 

PROVIDER: S-EPMC4651940 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature