Inhibiting the protein ubiquitination cascade by ubiquitin-mimicking short peptides.

ABSTRACT: Short heptapeptides were identified to function as ubiquitin (UB) mimics that are activated by E1 and form thioester conjugates with E1, E2, and HECT type E3 enzymes. The activities (k(cat)/K(1/2)) of E1 with the UB-mimicking peptides are 130-1,400-fold higher than the equally long peptide with the native C-terminal sequence of UB. By forming covalent conjugates with E1, E2, and E3 enzymes, the UB-mimicking peptides can block the transfer of native UB through the cascade.

SUBMITTER: Zhao B 

PROVIDER: S-EPMC4652583 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature