The synthetic genetic interaction network reveals small molecules that target specific pathways in Sacchromyces cerevisiae.

ABSTRACT: High-throughput elucidation of synthetic genetic interactions (SGIs) has contributed to a systems-level understanding of genetic robustness and fault-tolerance encoded in the genome. Pathway targets of various compounds have been predicted by comparing chemical-genetic synthetic interactions to a network of SGIs. We demonstrate that the SGI network can also be used in a powerful reverse pathway-to-drug approach for identifying compounds that target specific pathways of interest. Using the SGI network, the method identifies an indicator gene that may serve as a good candidate for screening a library of compounds. The indicator gene is selected so that compounds found to produce sensitivity in mutants deleted for the indicator gene are likely to abrogate the target pathway. We tested the uti

SUBMITTER: Tamble CM 

PROVIDER: S-EPMC4653045 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature