Nanoscale spatial organization of the HoxD gene cluster in distinct transcriptional states.

ABSTRACT: Chromatin condensation plays an important role in the regulation of gene expression. Recently, it was shown that the transcriptional activation of Hoxd genes during vertebrate digit development involves modifications in 3D interactions within and around the HoxD gene cluster. This reorganization follows a global transition from one set of regulatory contacts to another, between two topologically associating domains (TADs) located on either side of the HoxD locus. Here, we use 3D DNA FISH to assess the spatial organization of chromatin at and around the HoxD gene cluster and report that although the two TADs are tightly associated, they appear as spatially distinct units. We measured the relative position of genes within the cluster and found that they segregate over long distances, suggest

SUBMITTER: Fabre PJ 

PROVIDER: S-EPMC4653165 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature