Different Bla-g T cell antigens dominate responses in asthma versus rhinitis subjects.

ABSTRACT:

Background and objective

The allergenicity of several German cockroach (Bla-g) antigens at the level of IgE responses is well established. However, less is known about the specificity of CD4+ TH responses, and whether differences exist in associated magnitude or cytokine profiles as a function of disease severity.

Methods

Proteomic and transcriptomic techniques were used to identify novel antigens recognized by allergen-specific T cells. To characterize different TH functionalities of allergen-specific T cells, ELISPOT assays with sets of overlapping peptides covering the sequences of known allergens and novel antigens were employed to measure release of IL-5, IFNγ, IL-10, IL-17 and IL-21.

Results

Using these techniques, we characterized TH responses in a cohort of ad

SUBMITTER: Dillon MB 

PROVIDER: S-EPMC4654660 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature