Chemometric analysis of MALDI mass spectrometric images of three-dimensional cell culture systems.

ABSTRACT: As imaging mass spectrometry (IMS) has grown in popularity in recent years, the applications of this technique have become increasingly diverse. Currently there is a need for sophisticated data processing strategies that maximize the information gained from large IMS data sets. Traditional two-dimensional heat maps of single ions generated in IMS experiments lack analytical detail, yet manual analysis of multiple peaks across hundreds of pixels within an entire image is time-consuming, tedious and subjective. Here, various chemometric methods were used to analyze data sets obtained by matrix-assisted laser desorption/ionization (MALDI) IMS of multicellular spheroids. HT-29 colon carcinoma multicellular spheroids are an excellent in vitro model system that mimic the three dimensional

SUBMITTER: Weaver EM 

PROVIDER: S-EPMC4654961 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature