Visualizing viral protein structures in cells using genetic probes for correlated light and electron microscopy.

ABSTRACT: Structural studies of viral proteins most often use high-resolution techniques such as X-ray crystallography, nuclear magnetic resonance, single particle negative stain, or cryo-electron microscopy (EM) to reveal atomic interactions of soluble, homogeneous viral proteins or viral protein complexes. Once viral proteins or complexes are separated from their host's cellular environment, their natural in situ structure and details of how they interact with other cellular components may be lost. EM has been an invaluable tool in virology since its introduction in the late 1940's and subsequent application to cells in the 1950's. EM studies have expanded our knowledge of viral entry, viral replication, alteration of cellular components, and viral lysis. Most of these early studies were focused o

SUBMITTER: Ou HD 

PROVIDER: S-EPMC4655137 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature