The lh3 Glycosyltransferase Directs Target-Selective Peripheral Nerve Regeneration.

ABSTRACT: Functional PNS regeneration requires injured axons to return to their original synaptic targets, yet the mechanisms underlying target-selective regeneration have remained elusive. Using live-cell imaging in zebrafish we find that regenerating motor axons exhibit a strong preference for their original muscle territory and that axons probe both correct and incorrect trajectories extensively before selecting their original path. We show that this process requires the glycosyltransferase lh3 and that post-injury expression of lh3 in Schwann cells is sufficient to restore target-selective regeneration. Moreover, we demonstrate that Schwann cells neighboring the transection site express the lh3 substrate collagen4a5 and that during regeneration collagen4a5 destabilizes axons probing inappropriat

SUBMITTER: Isaacman-Beck J 

PROVIDER: S-EPMC4655140 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature