Use of genetically-encoded calcium indicators for live cell calcium imaging and localization in virus-infected cells.

ABSTRACT: Calcium signaling is a ubiquitous and versatile process involved in nearly every cellular process, and exploitation of host calcium signals is a common strategy used by viruses to facilitate replication and cause disease. Small molecule fluorescent calcium dyes have been used by many to examine changes in host cell calcium signaling and calcium channel activation during virus infections, but disadvantages of these dyes, including poor loading and poor long-term retention, complicate analysis of calcium imaging in virus-infected cells due to changes in cell physiology and membrane integrity. The recent expansion of genetically-encoded calcium indicators (GECIs), including blue and red-shifted color variants and variants with calcium affinities appropriate for calcium storage organelles like

SUBMITTER: Perry JL 

PROVIDER: S-EPMC4655165 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature