Quantitative activation suppression assay to evaluate human bone marrow-derived mesenchymal stromal cell potency.

ABSTRACT:

Background aims

With the increasing use of cell therapies involving immune modulatory cells, there is a need for a simple standardized method to evaluate and compare the suppressive potency of different cell products. We used the Karpas 299 (K299) cell line as the reference suppressor cell to develop a standardized suppression assay to quantify the immune-modulatory capacity of bone marrow-derived mesenchymal stromal cells (BM-MSCs).

Methods

Healthy donor CD4 T cells were co-cultured with the K299 cell line or with third-party BM-MSCs. After stimulation with anti-CD3/CD28 beads, CD154 activation and proliferation of CD4 T cells were measured to calculate suppression.

Results

The K299 cell line reproducibly suppressed both the activation and proliferation of healthy do

SUBMITTER: Salem B 

PROVIDER: S-EPMC4655179 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature