Identification of Bacillus strains by MALDI TOF MS using geometric approach.

ABSTRACT: Microorganism identification by MALDI TOF mass-spectrometry is based on the comparison of the mass spectrum of the studied organism with those of reference strains. It is a rapid and reliable method. However, commercial databases and programs are mostly designed for identification of clinically important strains and can be used only for particular mass spectrometer models. The need for open platforms and reference databases is obvious. In this study we describe a geometric approach for microorganism identification by mass spectra and demonstrate its capabilities by analyzing 24 strains belonging to the Bacillus pumilus group. This method is based on representing mass spectra as points on a multidimensional space, which allows us to use geometric distances to compare the spectra. Delimitati

SUBMITTER: Starostin KV 

PROVIDER: S-EPMC4655313 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature