A novel intrinsically fluorescent probe for study of uptake and trafficking of 25-hydroxycholesterol.

ABSTRACT: Cholesterol homeostasis is regulated not only by cholesterol, but also by oxygenated cholesterol species, referred to as oxysterols. Side-chain oxysterols, such as 25-hydroxycholesterol (25-HC), regulate cholesterol homeostasis through feedback inhibition and feed-forward activation of transcriptional pathways that govern cholesterol synthesis, uptake, and elimination, as well as through direct nongenomic actions that modulate cholesterol accessibility in membranes. Elucidating the cellular distribution of 25-HC is required to understand its biological activity at the molecular level. However, studying oxysterol distribution and behavior within cells has proven difficult due to the lack of fluorescent analogs of 25-HC that retain its chemical and physical properties. To address this, we sy

SUBMITTER: Iaea DB 

PROVIDER: S-EPMC4655980 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature