Effects of DNA methylation on expression of tumor suppressor genes and proto-oncogene in human colon cancer cell lines.

ABSTRACT:

Aim

To investigate the effects of DNA methylation on the expression of tumor suppressor genes and proto-oncogene in human colon cancer cell lines.

Methods

Three colon cancer cell lines (HT-29, SW1116 and Colo-320) treated with different concentrations of DNA methyltransferase inhibitor, 5-aza-2'-deoxycytidine (5-aza-dC) were used to induce DNA demethylation. The expressions of p16(INK4A), p21(WAF1), APC and c-myc genes were observed by using RT-PCR. The methylation status of p16(INK4A) promoter in HT-29 cells was also determined by methylation-specific PCR (MSP).

Results

Weak expressions of p16(INK4A) and APC in the three colon cancer cells were detected, and p21(WAF1) expression was not found in SW1116 and Colo-320 cells before treatment. After treatment of 1 micromo

SUBMITTER: Fang JY 

PROVIDER: S-EPMC4656656 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature