RNA-based, transient modulation of gene expression in human haematopoietic stem and progenitor cells.

ABSTRACT: Modulation of gene expression is a useful tool to study the biology of haematopoietic stem and progenitor cells (HSPCs) and might also be instrumental to expand these cells for therapeutic approaches. Most of the studies so far have employed stable gene modification by viral vectors that are burdensome when translating protocols into clinical settings. Our study aimed at exploring new ways to transiently modify HSPC gene expression using non-integrating, RNA-based molecules. First, we tested different methods to deliver these molecules into HSPCs. The delivery of siRNAs with chemical transfection methods such as lipofection or cationic polymers did not lead to target knockdown, although we observed more than 90% fluorescent cells using a fluorochrome-coupled siRNA. Confocal microscopic ana

SUBMITTER: Diener Y 

PROVIDER: S-EPMC4657003 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature