Bacterial metabolites directly modulate farnesoid X receptor activity.

ABSTRACT:

Background

The farnesoid X receptor (FXR), a ligand-activated transcription factor belonging to the adopted orphan receptor, plays an important role in maintaining health of the liver and intestine. In this study, we identified individual bacterial strains that directly modulated the activation of intestinal FXR.

Methods

The FXR stimulatory potential of 38 bacterial strains was determined using a stable FXR reporter system derived from intestinal epithelial cells (IEC). The induction of FXR target genes by screened FXR stimulatory bacteria was determined by real-time PCR. In addition, a high fat diet (HFD)-induced obese mouse model was used to evaluate in vivo FXR stimulatory potential of bacterial metabolites screened in this study.

Results

A luciferase assay with th

SUBMITTER: Zhang X 

PROVIDER: S-EPMC4657204 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature