Annotation of pseudogenic gene segments by massively parallel sequencing of rearranged lymphocyte receptor loci.

ABSTRACT:

Background

The adaptive immune system generates a remarkable range of antigen-specific T-cell receptors (TCRs), allowing the recognition of a diverse set of antigens. Most of this diversity is encoded in the complementarity determining region 3 (CDR3) of the β chain of the αβ TCR, which is generated by somatic recombination of noncontiguous variable (V), diversity (D), and joining (J) gene segments. Deletion and non-templated insertion of nucleotides at the D-J and V-DJ junctions further increases diversity. Many of these gene segments are annotated as non-functional owing to defects in their primary sequence, the absence of motifs necessary for rearrangement, or chromosomal locations outside the TCR locus.

Methods

We sought to utilize a novel method, based on high-throughpu

SUBMITTER: Dean J 

PROVIDER: S-EPMC4657264 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature