A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.

ABSTRACT:

Background

Aortic diseases are diverse and involve a multiplicity of biological systems in the vascular wall. Aortic dissection, which is usually preceded by aortic aneurysm, is a leading cause of morbidity and mortality in modern societies. Although the endothelium is now known to play an important role in vascular diseases, its contribution to aneurysmal aortic lesions remains largely unknown. The aim of this study was to define a reliable methodology for the isolation of aortic intimal and adventitial endothelial cells in order to throw light on issues relevant to endothelial cell biology in aneurysmal diseases.

Methodology/principal findings

We set up protocols to isolate endothelial cells from both the intima and the adventitia of human aneurysmal aortic vessel segments

SUBMITTER: Leclercq A 

PROVIDER: S-EPMC4658207 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature