Revisiting the role of phospholipases C in virulence and the lifecycle of Mycobacterium tuberculosis.

ABSTRACT: Mycobacterium tuberculosis, the agent of human tuberculosis has developed different virulence mechanisms and virulence-associated tools during its evolution to survive and multiply inside the host. Based on previous reports and by analogy with other bacteria, phospholipases C (PLC) of M. tuberculosis were thought to be among these tools. To get deeper insights into the function of PLCs, we investigated their putative involvement in the intracellular lifestyle of M. tuberculosis, with emphasis on phagosomal rupture and virulence, thereby re-visiting a research theme of longstanding interest. Through the construction and use of an M. tuberculosis H37Rv PLC-null mutant (ΔPLC) and control strains, we found that PLCs of M. tuberculosis were not required for induction of phagosomal rupture and o

SUBMITTER: Le Chevalier F 

PROVIDER: S-EPMC4658479 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature