RNA Futile Cycling in Model Persisters Derived from MazF Accumulation.

ABSTRACT:

Unlabelled

Metabolism plays an important role in the persister phenotype, as evidenced by the number of strategies that perturb metabolism to sabotage this troublesome subpopulation. However, the absence of techniques to isolate high-purity populations of native persisters has precluded direct measurement of persister metabolism. To address this technical challenge, we studied Escherichia coli populations whose growth had been inhibited by the accumulation of the MazF toxin, which catalyzes RNA cleavage, as a model system for persistence. Using chromosomally integrated, orthogonally inducible promoters to express MazF and its antitoxin MazE, bacterial populations that were almost entirely tolerant to fluoroquinolone and β-lactam antibiotics were obtained upon MazF accumulation, and

SUBMITTER: Mok WW 

PROVIDER: S-EPMC4659464 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature