Automated Classification and Cluster Visualization of Genotypes Derived from High Resolution Melt Curves.

ABSTRACT:

Introduction

High Resolution Melting (HRM) following PCR has been used to identify DNA genotypes. Fluorescent dyes bounded to double strand DNA lose their fluorescence with increasing temperature, yielding different signatures for different genotypes. Recent software tools have been made available to aid in the distinction of different genotypes, but they are not fully automated, used only for research purposes, or require some level of interaction or confirmation from an analyst.

Materials and methods

We describe a fully automated machine learning software algorithm that classifies unknown genotypes. Dynamic melt curves are transformed to multidimensional clusters of points whereby a training set is used to establish the distribution of genotype clusters. Subsequently, prob

SUBMITTER: Kanderian S 

PROVIDER: S-EPMC4659556 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature