Toxicity Minimized Cryoprotectant Addition and Removal Procedures for Adherent Endothelial Cells.

ABSTRACT: Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required to suppress ice formation. Recently, we described a mathematical strategy for identifying minimally toxic CPA equilibration procedures based on the minimization of a toxicity cost function. Here we provide direct experimental support for the feasibility of these methods when applied to adherent endothelial cells. We first developed a concentration- and temperature-dependent toxicity cost function by exposing the cells to a r

SUBMITTER: Davidson AF 

PROVIDER: S-EPMC4659675 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature