Protein structural dynamics revealed by time-resolved X-ray solution scattering.

ABSTRACT: One of the most important questions in biological science is how a protein functions. When a protein performs its function, it undergoes regulated structural transitions. In this regard, to better understand the underlying principle of a protein function, it is desirable to monitor the dynamic evolution of the protein structure in real time. To probe fast and subtle motions of a protein in physiological conditions demands an experimental tool that is not only equipped with superb spatiotemporal resolution but also applicable to samples in solution phase. Time-resolved X-ray solution scattering (TRXSS), discussed in this Account, fits all of those requirements needed for probing the movements of proteins in aqueous solution. The technique utilizes a pump-probe scheme employing an optical pu

SUBMITTER: Kim JG 

PROVIDER: S-EPMC4659713 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature