DNA damage induces a meiotic arrest in mouse oocytes mediated by the spindle assembly checkpoint.

ABSTRACT: Extensive damage to maternal DNA during meiosis causes infertility, birth defects and abortions. However, it is unknown if fully grown oocytes have a mechanism to prevent the creation of DNA-damaged embryos. Here we show that DNA damage activates a pathway involving the spindle assembly checkpoint (SAC) in response to chemically induced double strand breaks, UVB and ionizing radiation. DNA damage can occur either before or after nuclear envelope breakdown, and provides an effective block to anaphase-promoting complex activity, and consequently the formation of mature eggs. This contrasts with somatic cells, where DNA damage fails to affect mitotic progression. However, it uncovers a second function for the meiotic SAC, which in the context of detecting microtubule-kinetochore errors has hi

SUBMITTER: Collins JK 

PROVIDER: S-EPMC4659839 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature