Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves.

ABSTRACT: In single-cell analysis, cellular activity and parameters are assayed on an individual, rather than population-average basis. Essential to observing the activity of these cells over time is the ability to trap, pattern and retain them, for which previous single-cell-patterning work has principally made use of mechanical methods. While successful as a long-term cell-patterning strategy, these devices remain essentially single use. Here we introduce a new method for the patterning of multiple spatially separated single particles and cells using high-frequency acoustic fields with one cell per acoustic well. We characterize and demonstrate patterning for both a range of particle sizes and the capture and patterning of cells, including human lymphocytes and red blood cells infected by the mala

SUBMITTER: Collins DJ 

PROVIDER: S-EPMC4659840 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature