A High-Throughput Regeneration and Transformation Platform for Production of Genetically Modified Banana.

ABSTRACT: Banana (Musa spp.) is an important staple food as well as cash crop in tropical and subtropical countries. Various bacterial, fungal, and viral diseases and pests such as nematodes are major constraints in its production and are currently destabilizing the banana production in sub-Saharan Africa. Genetic engineering is a complementary option used for incorporating useful traits in banana to bypass the long generation time, polyploidy, and sterility of most of the cultivated varieties. A robust transformation protocol for farmer preferred varieties is crucial for banana genomics and improvement. A robust and reproducible system for genetic transformation of banana using embryogenic cell suspensions (ECS) has been developed in this study. Two different types of explants (immature male flower

SUBMITTER: Tripathi JN 

PROVIDER: S-EPMC4659906 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature