Dataset Information

The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.

ABSTRACT: Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50-90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3-74.6 °C), the microbial communities were dominated by Bacteria, which accounted for 60-80% of the total number of cells. At 74.6 °C, Archaea were dominant, and at 90.8 °C, they accounted for more than 90% of the total number of cells. Additionally, the microbial communities at high temperatures (74.6-90.8 °C) were substantially simpler than those at the low-temperature sites. Only a few genera (e.g., bacterial Caldisericum, Thermotoga and Thermoanaerobacter, archaeal Vulcanisaeta and Hyperthermus) often dominated in high-temperature environments. Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected. AOA activity increased from 17 to 52 pmol of NO2(-) per cell d(-1) with a temperature change from 50 to 70 °C.

SUBMITTER: Li H

PROVIDER: S-EPMC4660298 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.

Li Haizhou H Yang Qunhui Q Li Jian J Gao Hang H Li Ping P Zhou Huaiyang H

Scientific reports 20151126

Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50-90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3-74.6 °C), the microbial communities were dominated by Bacteria, which ...[more]

PMID: 26608685

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Dataset Information

The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.

Publications

The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China.

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