TAL effectors mediate high-efficiency transposition of the piggyBac transposon in silkworm Bombyx mori L.

ABSTRACT: The piggyBac (PB) transposon is one of the most useful transposable elements, and has been successfully used for genetic manipulation in more than a dozen species. However, the efficiency of PB-mediated transposition is still insufficient for many purposes. Here, we present a strategy to enhance transposition efficiency using a fusion of transcription activator-like effector (TALE) and the PB transposase (PBase). The results demonstrate that the TALE-PBase fusion protein which is engineered in this study can produce a significantly improved stable transposition efficiency of up to 63.9%, which is at least 7 times higher than the current transposition efficiency in silkworm. Moreover, the average number of transgene-positive individuals increased up to 5.7-fold, with each positive brood con

SUBMITTER: Ye L 

PROVIDER: S-EPMC4660427 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature