Cell-secreted matrices perpetuate the bone-forming phenotype of differentiated mesenchymal stem cells.

ABSTRACT: Prior to transplantation, mesenchymal stem/stromal cells (MSCs) can be induced toward the osteoblastic phenotype using a cocktail of soluble supplements. However, there is little evidence of differentiated MSCs directly participating in bone formation, suggesting that MSCs may either die or revert in phenotype upon transplantation. Cell-secreted decellularized extracellular matrices (DMs) are a promising platform to confer bioactivity and direct cell fate through the presentation of a complex and physiologically relevant milieu. Therefore, we examined the capacity of biomimetic DMs to preserve the mineral-producing phenotype upon withdrawal of the induction stimulus. Regardless of induction duration, ranging up to 6 weeks, MSCs exhibited up to a 5-fold reduction in osteogenic markers withi

SUBMITTER: Hoch AI 

PROVIDER: S-EPMC4661076 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature