Co-fabrication of chitosan and epoxy photoresist to form microwell arrays with permeable hydrogel bottoms.

ABSTRACT: Microfabrication technology offers the potential to create biological platforms with customizable patterns and surface chemistries, allowing precise control over the biochemical microenvironment to which a cell or group of cells is exposed. However, most microfabricated platforms grow cells on impermeable surfaces. This report describes the co-fabrication of a micropatterned epoxy photoresist film with a chitosan film to create a freestanding array of permeable, hydrogel-bottomed microwells. These films possess optical properties ideal for microscopy applications, and the chitosan layers are semi-permeable with a molecular exclusion of 9.9 ± 2.1 kDa. By seeding cells into the microwells, overlaying inert mineral oil, and supplying media via the bottom surface, this hybrid film permits cell

SUBMITTER: Ornoff DM 

PROVIDER: S-EPMC4661114 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature