A New Zamilon-like Virophage Partial Genome Assembled from a Bioreactor Metagenome.

ABSTRACT: Virophages replicate within viral factories inside the Acanthamoeba cytoplasm, and decrease the infectivity and replication of their associated giant viruses. Culture isolation and metagenome analyses have suggested that they are common in our environment. By screening metagenomic databases in search of amoebal viruses, we detected virophage-related sequences among sequences generated from the same non-aerated bioreactor metagenome as recently screened by another team for virophage capsid-encoding genes. We describe here the assembled partial genome of a virophage closely related to Zamilon, which infects Acanthamoeba with mimiviruses of lineages B and C but not A. Searches for sequences related to amoebal giant viruses, other Megavirales representatives and virophages were conducted using

SUBMITTER: Bekliz M 

PROVIDER: S-EPMC4661282 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature