Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.

ABSTRACT: Homing endonucleases recognize and generate a DNA double-strand break, which has been used to promote gene targeting. These enzymes recognize long DNA stretches; they are highly sequence-specific enzymes and display a very low frequency of cleavage even in complete genomes. Although a large number of homing endonucleases have been identified, the landscape of possible target sequences is still very limited to cover the complexity of the whole eukaryotic genome. Therefore, the finding and molecular analysis of homing endonucleases identified but not yet characterized may widen the landscape of possible target sequences. The previous characterization of protein-DNA interaction before the engineering of new homing endonucleases is essential for further enzyme modification. Here we report the

SUBMITTER: Molina R 

PROVIDER: S-EPMC4661390 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature